Bahram Falahatkar; Khayam Delafkar; Masoud Sattari; Hossein Khara
Volume 5, Issue 3 , March 2017, , Pages 79-96
Abstract
The present study was carried out to investigate the efficacy of two anesthetics, clove oil and ketamine, comparison of hematological parameters, and blood biochemical indices under optimum doses of the two anesthetics in juvenile Sterlet Acipenser ruthenus (n=276) with an average weight of 64.1 ± ...
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The present study was carried out to investigate the efficacy of two anesthetics, clove oil and ketamine, comparison of hematological parameters, and blood biochemical indices under optimum doses of the two anesthetics in juvenile Sterlet Acipenser ruthenus (n=276) with an average weight of 64.1 ± 3.4 g. Clove oil was used in concentrations of 14, 35, 56, 77, 98 and 119 mg/l, and those of 35, 37.5, 40, 42.5, 45 and 50 mg/l for ketamine in three replicates. Both losing time of equilibrium and induced time of anesthesia were reduced by increasing the concentrations of these two agents, whereas time of recovery increased. Optimum doses regarding the time of anesthesia and recovery were obtained as 56 mg/l and 45 mg/l for clove oil and ketamine, respectively. There were significant decreases in hematological parameters for all treatments compared to control group (P<0.05). No significant changes were observed in cortisol, glucose, and lactate concentrations for fish treated with clove oil (P>0.05), whereas, fish exposed to handling stress showed significant changes in all biochemical indices (P<0.05). On the other hand, glucose concentration did not change significantly in ketamine treatment (P>0.05), but cortisol and lactate changed significantly (P<0.05). Considering appropriate efficacy of clove oil in both induction and recovery times, and rendering no changes in biochemical indices, clove oil would be recommended as an alternative to ketamine for this species during aquaculture practices.
Hosein Khara; Marjan Pouresmaeilian; Mohaddese Ahmadnezhad
Volume 5, Issue 1 , September 2016, , Pages 9-22
Abstract
Reproductive biology of female Shemaya (Alburnus chalcoides) studied with examination of histology, egg diameter and value of sex steroid hormones during spawning phase in the Anzali wetland from March 2013 to Jun 2014. Finally 42 numbers of female fish were examined. The result of sex steroid hormones ...
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Reproductive biology of female Shemaya (Alburnus chalcoides) studied with examination of histology, egg diameter and value of sex steroid hormones during spawning phase in the Anzali wetland from March 2013 to Jun 2014. Finally 42 numbers of female fish were examined. The result of sex steroid hormones showed the rate of testosterone hormone in gravid fish was 7.93±1.18 ng/ml; but in ripe females, its value was 3.88±1.12 ng/ml (P<0.05). Level of 17β- estradiol during spawning phase were examined and its value in gravid fish and ripe fish respectively was 2.98±0.18 and 2.55±0.09 ng/ml. also level of progesterone hormone in ripe fish was 0.36±0.04 ng/ml, that its value in ripe fish and its value in gravid fish was 0.25±0.03 ng/ml (P<0.05). Egg diameter in gravid fish and ripe fish respectively was 655.12±13.29 and 792.4±13.96 micron. Microscopic study of ovarian histology in female fish showed ovarian contain eggs in primary growth stage, cortical alveoli, early vitellogenic oocyte, advanced vitellogenic oocyte, hydrated oocyte, germinal visicol break down oocyte. According to value of 17β-estradiol during spawning phase and result of other study, probably eggs are growing during spawning phase. The process of oocyte growth in these fishes is asynchronous.
